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. Author manuscript; available in PMC: 2008 Nov 25.
Published in final edited form as: Nature. 2008 Apr 24;452(7190):961–965. doi: 10.1038/nature06889

Figure 2. Crystal structures of AlkB–DNA4 and AlkB–DNA5 complexes with 1-meA recognized by an intact active site.

Figure 2

a, A strategy to set up a disulphide cross-link away from the active site of AlkB. b, Cartoon of the AlkB–DNA4 complex with 1-meA (light magenta) recognized in the active site of AlkB. The complex was stabilized by a disulphide link between an engineered Cys 129 (green) and C10* that is two base pairs away from 1-meA. The colour coding is the same as in Fig. 1b. DNA4 has an abasic site ‘ٮ’ (position 7′) 3′ to T8′ of the 1-meA:T base pair. c, Cartoon trace of the AlkB–DNA5 complex; DNA5 has a central 1-meA:A base pair replacing a C*:A in DNA1 and a C10* cross-linking site two base pairs away from 1-meA.