FIG. 4.

Effect of human Ape1 and Cys65 redox Ape1 mutant on NF-κB and AP-1 transactivation in a cell-based reporter assay system. SKOV-3X cells were transfected with AP-1 or NF-κB–Luc construct containing an NF-κB or AP-1–response promoter and driving the expression of a luciferase gene. The cells were cotransfected with plasmid pcDNA–wild-type hApe1 or the redox mutant (pcDNA-C65A) and a Renilla luciferase control reporter vector pRL-CMV. After a 24-h transfection period, cells were lysed, and Firefly and Renilla luciferase activities were assayed by using Renilla luciferase activity for normalization. All of the transfection experiments were performed in triplicate and repeated at least 3 times in independent experiments. Data are expressed as mean ± standard error from a representative experiment, and Student's t tests were performed. *Significant difference at the p < 0.05 level comparing the Cys65 mutant and wt-Ape1 and wt-Ape1 and mock or pcDNA.