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. Author manuscript; available in PMC: 2009 Jun 1.

Published in final edited form as: Mol Cancer Ther. 2008 Jun;7(6):1506–1513. doi: 10.1158/1535-7163.MCT-07-2334

In cells where HIF-1α is down-regulated by siRNA, CCI-779 does not increase the toxicity of 2-DG in hypoxic cells. (A) Westernblot analysis of SCLC line B treated under normoxic (N) and hypoxic (H) conditions confirming the effectiveness of siRNA in down-regulating HIF-1α. (B, C) Under hypoxia, CCI-779 does not increase 2-DG toxicity in cells transfected with siRNA directed against HIF-1α. In contrast, CCI-779 increases 2-DG toxicity in cells treated with scrambled RNA. Each data point is the average of triplicate samples with S.D. *P=0.07, **p=0.016

In cells where HIF-1α is down-regulated by siRNA, CCI-779 does not increase the toxicity of 2-DG in hypoxic cells. (A) Westernblot analysis of SCLC line B treated under normoxic (N) and hypoxic (H) conditions confirming the effectiveness of siRNA in down-regulating HIF-1α. (B, C) Under hypoxia, CCI-779 does not increase 2-DG toxicity in cells transfected with siRNA directed against HIF-1α. In contrast, CCI-779 increases 2-DG toxicity in cells treated with scrambled RNA. Each data point is the average of triplicate samples with S.D. *P=0.07, **p=0.016