TABLE 4.
PLP-catalyzed formation of pyruvate and ammonium from THT-Aa
| Time (h) | Pyruvate (nmol) | Ammonium (nmol) | |
|---|---|---|---|
| No PLP | 0 | 47.6±1.0 | 48.8±2.4 |
| 1 | 65.0±2.6 | 63.0±6.4 | |
| 2 | 88.0±4.0 | 84.8±3.8 | |
| Plus PLPb | 0 | 51.0±1.0 | 60.4±12.2 |
| 1 | 168±1 | 185±9 | |
| 2 | 248±3 | 264±6 |
a
The reaction mixture (0.2 mL) contained 100 mM potassium phosphate buffer pH 7.4, 5 mM THT-A, and where indicated 2.5 μM PLP. After incubation at 37 °C at the times indicated, 10-μL aliquots were analyzed for pyruvate and ammonium (n = 3).
b
The blank contained PLP and phosphate buffer, but no THT-A.