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. Author manuscript; available in PMC: 2008 Nov 25.
Published in final edited form as: Nature. 2007 May 21;447(7148):1116–1120. doi: 10.1038/nature05894

Figure 4.

Figure 4

Impaired glucose homeostasis in high fat fed male Mac-PPARγ KO mice. a, Oral glucose tolerance tests (1 g/kg) in male mice after 19 weeks of feeding the HFD (n=5/genotype). b, Insulin tolerance test. Obese mice were fasted for 4 hours prior to intraperitoneal injection of insulin (0.65 u/kg). c, Fasting serum insulin levels in control and Mac-PPARγ KO mice after 4 hr fast. d, Homa-IR index of insulin sensitivity (insulin [ng/ml] x glucose [mM]). e-f, Decreased insulin signaling in obese Mac-PPARγ KO mice. Control and Mac-PPARγ KO mice were injected with insulin (5 mU/g), and cellular lysates were immunoblotted for total and serine phosphorylated (S473) Akt; liver (e) and quadriceps (f). g-h, Relative transcript levels of genes involved in β-oxidation and oxidative phosphorylation, and of transcriptional regulators controlling these pathways in quadriceps (g) and liver (h). i, Circulating levels of adiponectin in control and Mac-PPARγ KO mice.