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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: J Immunol. 2008 Aug 1;181(3):1835–1848. doi: 10.4049/jimmunol.181.3.1835

FIGURE 8. In vivo depletion of Tregs in old L. major-infected mice increases the production of IFN-γ by Teffs at the infection site.

FIGURE 8

(A) Old L. major-infected mice (> 48 week old, which had been infected when they were 8-week old) or young L. major-infected mice (16-week old, which had been infected when they were 8-week old) were treated with anti-CD25 Ab or isotype control (N = 4/group) (1 mg for 3 weeks, twice a week). After Ab treatment, mice were sacrificed. T cells were purified from the infection site and draining LNs and restimulated in vitro with L. major-infected BMDCs. (B) IFN-γ and IL-10 were measured by ELISA in 4-day supernatants. P values correspond to the comparisons between mice treated with isotype control (open symbols) or anti-CD25 Ab (hatched symbols) using t-tests.