(A) Map of the HIS4LEU2 locus showing diagnostic restriction sites and position of the probe. DNA species detected following Southern hybridization are shown below. SEI-1 and SEI-2 are the two major SEI species detected with Probe 4 (Hunter and Kleckner, 2001). Lollipops indicate restriction sites: X, XhoI.
(B) Image of one-dimensional (1D) Southern analysis showing DNA species detailed in (A).
(C) Predicted structures of SEI and dHJ joint molecule intermediates.
(D) Image of a native/native 2D analysis. Species detailed in (A) are highlighted.