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. Author manuscript; available in PMC: 2009 Aug 8.
Published in final edited form as: Mol Cell. 2008 Aug 8;31(3):324–336. doi: 10.1016/j.molcel.2008.07.006

Figure 2. Physical Assay System for Monitoring Recombination.

Figure 2

(A) Map of the HIS4LEU2 locus showing diagnostic restriction sites and position of the probe. DNA species detected following Southern hybridization are shown below. SEI-1 and SEI-2 are the two major SEI species detected with Probe 4 (Hunter and Kleckner, 2001). Lollipops indicate restriction sites: X, XhoI.

(B) Image of one-dimensional (1D) Southern analysis showing DNA species detailed in (A).

(C) Predicted structures of SEI and dHJ joint molecule intermediates.

(D) Image of a native/native 2D analysis. Species detailed in (A) are highlighted.