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. Author manuscript; available in PMC: 2009 Jul 15.

Published in final edited form as: Anal Biochem. 2008 Apr 7;378(2):177–183. doi: 10.1016/j.ab.2008.04.007

UV-Vis difference spectra (spectrum of Co-L1 minus spectrum of apo-L1) of cobalt-containing analogs of L1: (top) cobalt-containing L1 prepared by biological incorporation and (bottom) refolded, cobalt-containing L1. The enzyme concentration in all samples was 550 µM, and the buffer was 50 mM cacocylate, pH 7.0.

UV-Vis difference spectra (spectrum of Co-L1 minus spectrum of apo-L1) of cobalt-containing analogs of L1: (top) cobalt-containing L1 prepared by biological incorporation and (bottom) refolded, cobalt-containing L1. The enzyme concentration in all samples was 550 µM, and the buffer was 50 mM cacocylate, pH 7.0.