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. Author manuscript; available in PMC: 2009 Jul 15.
Published in final edited form as: Anal Biochem. 2008 Apr 7;378(2):177–183. doi: 10.1016/j.ab.2008.04.007

Figure 4.

Figure 4

Figure 4

Figure 4

Stopped-flow fluorescence (A) progress curve for reaction of refolded 2Co-L1 and nitrocefin. UV-Vis stopped-flow progress curves on (B) refolded 2Co-L1 and (C) 2Zn-L1 reacted with nitrocefin. The reactions were carried out in Chelex-treated, 50 mM cacodylate, pH 7.0, at 10 °C, and the enzyme and substrate concentrations were 50 µM.