. Author manuscript; available in PMC: 2009 Jul 15.

Published in final edited form as: Anal Biochem. 2008 Apr 7;378(2):177–183. doi: 10.1016/j.ab.2008.04.007

Table 1.

Characterization of Co(II)-substituted L1 analogs.

Species	Cobalt content (eq)	k_cat (s⁻¹)	K_m (µM)
Biologicallyincorporated L1	1.0 ± 0.1	2.8 ± 0.1	13 ± 2
1 CoL1	1	14 ± 1	10 ± 3
2 CoL1	2	27± 1	8 ± 1
3 CoL1	3	71± 9	30± 7
L1 refolded w Co(II)	1.8 ± 0.1	63 ± 3	20 ± 1

Steady state kinetic studies were conducted in Chelex-treated 50 mM cacodylate buffer, pH 7.0, using nitrocefin as the substrate.