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Cy3-labeled BDNF siRNA applied to the cut axons is transported retrogradely to the RGC cell bodies. A. At 3 days after axotomy, the GCL of a flat-mounted retina shows punctate accumulations of Cy3-labeled siRNA in RGCs. B. The GCL of a control retina with PBS application only shows only background fluorescence. Scale bar in B: 50 μm.
