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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: J Immunol. 2008 Aug 1;181(3):2044–2055. doi: 10.4049/jimmunol.181.3.2044

Figure 3. CpG-B DNA induces activation of PKD1 but not PKD2 and PKD3.

Figure 3

RAW264.7 cells stably expressing empty vector, FLAG-tagged PKD1, FLAG-tagged PKD2, or FLAG-tagged PKD3 were stimulated with medium, CpG-B DNA (12 μg/ml) for 45 min. Each PKD family protein in whole cell lysates was immunoprecipitated with anti-FLAG Ab. Kinase activity of PKDs was analyzed by in vitro kinase assay using syntide-2 as a PKD substrate (top). Expression and phosphorylation status of each PKD was analyzed by immunoblotting with anti-FLAG and anti-phospho-PKD Abs, respectively (bottom).