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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: J Immunol. 2008 Aug 1;181(3):2044–2055. doi: 10.4049/jimmunol.181.3.2044

Figure 7. Src PTKs, PI3K, PLC-γ and PKCs are not required for CpG-B DNA-mediated PKD1 activation.

Figure 7

A and B, RAW264.7 cells pretreated with DMSO or Src PTK inhibitors (PP1 or SU6656) for 30 min (A), or that constitutively over-express empty vector or mCsk (B) were stimulated with medium or CpG-B DNA (12 μg/ml) for 45 min. C–E, RAW264.7 cells were pretreated with DMSO, PI3K inhibitor (LY294002), PLC-γ inhibitor (U73122), PKC inhibitors (Gö6850, Gö6976 or Gö6983 at 125 ng/ml), or EGTA for 30 min and then stimulated with medium, CpG-B DNA, or PMA for 45 min. Phosphorylation status of PKD1 and PTKs was detected by Western blot using a phospho-PKD specific antibody and phospho-tyrosine specific antibody (4G10), respectively. med: medium, SU: SU6656, LY: LY294002, U73: U73122.