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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: J Immunol. 2008 Aug 1;181(3):2044–2055. doi: 10.4049/jimmunol.181.3.2044

Figure 9. PKD1 interacts with TLR9 signaling complex in response to CpG-B DNA.

Figure 9

RAW264.7 cells stably expressing empty vector, FLAG-tagged PKD1, FLAG-tagged PKD2, or FLAG-tagged PKD3 were stimulated with medium (med) or CpG-B DNA for 30 min. A, FLAG-tagged PKD family proteins in the cell lysates were immunoprecipitated. The presence of proteins in the TLR9-signaling pathway and phosphorylated forms of PKD in the resulting immunoprecipitates were detected by Western blot. B, The indicated endogenous proteins in the TLR9-signaling pathway in the cell lysates were immunoprecipitated. The presence of FLAG-tagged PKD family proteins and proteins in the TLR9-signaling pathway in the resulting immunoprecipitates were detected by Western blot.