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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: J Immunol. 2008 Aug 1;181(3):2044–2055. doi: 10.4049/jimmunol.181.3.2044

Table 1.

Sequences of primers

Gene Forward/sense primer Reverse/anti-sense primer
Cloning PKD1 ATGAGCGTCCCTCCGCTGCT GAGGATGCTGACACGCTCAC
PKD2 ATGGCCGCCGCCCCCTCCCAT GAGGATGCTGATGCGCTCAG
PKD3 ATGTCTGCAAATAATTCCCCTC AGGATCCTCCTCCATGTCGT
shRNA Luciferase GACAGTGTGGCATTTATTA TAATAAATGCCACACTGTC
PKD1 802 CTCCTGATGTCTAAGGTGA TCACCTTAGACATCAGGAG
PKD1 2453 CCATTGATCTTATCAATAA TTATTGATAAGATCAATGG
IRAK1 845 GCTTATACTGCCTTGTTTA TAAACAAGGCAGTATAAGC
RT- PCR TNF-α CGATGGGTTGTACCTTGTCTACTC TGGAAGACTCCTCCCAGGTATATG
IL-6 AAGTCCGGAGAGGAGACTTCACAG GTCTTGGTCCTTAGCCACTCCTTC
IL-10 TATGCTGCCTGCTCTTACTGACTG TTCATGGCCTTGTAGACACCTTGG
IL-12 p40 AGACCCTGACCATCACTGTCAAAG GAAGAAGCTGGTGCTGTAGTTCTC
* IFN-α ATGGCTAGRCTCTGTGCTTTCCT AGGGCTCTCCAGAYTTCTGCTCTG
* IFN-β CATCAACTATAAGCAGCTCCA TTCAAGTGGAGAGCAGTTGAG
GAPDH GTCTTCACCACCATGGAGAAGGC GGCATGGACTGTGGTCATGA
PKD1 CGGTACTGTCCGCACAGCAT ATTCTGCACCAGGGCTAAGTAAT
PKD2 ATTCTGTGCGCCTTGGCAG GCTTCCTCCATCGGCACG
PKD3 GCCAGGACCTGGCCTCTC GGGCATGTCTGCATCAGTC
PKCα AAATCGCCGACTTTGGGATG TTGGGCTTGAATGGTGGTTG
PKCε GGCTCGGAAACACCCTTATC CAAGTCGTCCTCGTTGTCAG
PKCζ CTGTCATGCCTTCCCAAGAG CTGGAAGCAGGAGTGTAAGC
TLR9 AGCTGAACATGAACGGCATCT AAAACTGAAATTGTGGCCTA
MyD88 CCCAACGATATCGAGTTTGT TTCTTCATCGCCTTGTATTT
IRAK1 ATGGCAGCCTCTAGTAGTGA CTATCCAAGACCCCTTCTTC
IRAK4 CAAAATATCTGACTTTGGGC TAGTTGAGGTTCACGGTTTT
TRAF6 AGGCAGGTTTCTTGTGTAAA ATGTGCAACTGGGTATTCTC
Actin AAGGCCAACCGTGAAAAGATGACC ACCGCTCGTTGCCAATAGTGATGA
hIL-6 ACTCACCTCTTCAGAACGAA CTCAAACTCCAAAAGACCAG
hIL-8 CACCCCAAATTTATCAAAGA TCAAAAACTTCTCCACAACC
hActin GTGGGGCGCCCCAGGCACCA CTCCTTAATGTCACGCACGATTTC
*

: Primer sequences of IFN-α and -β were described previo/usly (58)