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. 2000 May 2;97(10):5598–5603. doi: 10.1073/pnas.100104597

Figure 4.

Figure 4

Fear conditioning in Syt IV mutant mice. (A) Twenty-four-hour context test in wild type (n = 10) and Syt IV mutants (n = 15) after a single foot shock (0.75 mA for 2 s). Mean percentage of freezing during each minute of the 4-min context test is shown. Data points and bars represent mean ± SEM, respectively. MANOVA indicated a significant main effect of genotype [F (1, 23) = 7.6; P = 0.01]. (B) The trained and tested mice from A were given three additional foot shocks, separated by 60 s, and then tested 24 h later as above. MANOVA revealed no significant effect of genotype [F (1, 22) = 2.6; P > 0.1]. (C) Twenty-four-hour cued conditioning in another group of wild-type (n = 15) and Syt IV mutant (n = 14) mice. Mice were trained as above with a 10-s tone (90 dB; 2.8 kHz) coterminated with a single foot shock and subsequently tone tested in a novel context. Mean percentage of freezing during a 2-min baseline period before tone and for 3 min immediately after tone are shown. Data points and bars represent mean ± SEM, respectively. MANOVA revealed no significant effect of genotype [F (1, 27) = 0.01; P = 0.9] and a significant genotype-by-time interaction [F (4, 108) = 3.2; P < 0.02]. There was a significant effect of genotype during the second minute of baseline period [F (1, 27) = 4.2; P < 0.05]. There were no significant differences at any other minute.