Fig. 1.

Experimental design to study the stage specific effect of dietary silibinin feeding on prostate tumor progression, invasion, migration and metastasis in TRAMP mice. Male TRAMP mice starting at 4, 12, 20 and 30 weeks of age were fed with control or 1% silibinin-supplemented [1% silibinin (w/w) in AIN-93M purified] diet and then sacrificed at 12, 20, 30 and 45 weeks of age, respectively. The different groups depending upon their study period were referred to as the 4-12, 12-20, 20-30 and 30-45 week groups, respectively. Sb, silibinin.