Table 1.

Stage specific effect of silibinin feeding on prostate tumor metastasis to lungs, liver and kidney in TRAMP mice

Treatment time (weeks)	% incidence of metastatic lesions
	Lung		Liver		Kidney
	Control	1% Sb-fed	Control	1% Sb-fed	Control	1% Sb-fed
4-12	0	0	0	0	0	0
12-20	10	0	6	0	6	0
20-30	78	24*	34	14	50	10#
30-45	100	72	75	57	88	64

In the experiment detailed in Fig.1, at the time of necropsy any evidence of gross metastatic lesions in non-target organs was noted. H& E stained tissue sections of lung, liver and kidney of positive controls and their respective silibinin-fed groups were microscopically analyzed for metastatic lesions. Values are shown as percent of mice having metastatic lesions in particular organ for each group. Fisher’s Exact test was used to compare the incidence of metastatic lesions in positive control versus silibinin-fed groups. The numbers of animals in 4-12, 12-20, 20-30 and 30-45 week groups were 17, 20, 15 and 22 in the positive control groups; and 22, 18, 16 and 15 in the 1% Sb-fed groups, respectively. P values <0.05 were considered significant.

^*P<0.001;

^#P<0.01.

Control, positive control (TRAMP mice); Sb, silibinin.