Figure 2.
(A) & (B) Nitration status of p53 was studied by immunoprecipitation analysis in Alzheimer’s disease (AD), mild cognitive impairment (MCI) and control IPL. Equal amounts of protein (150 µg/lane) were immunoprecipitated by anti-p53 antibody, and immunoprecipitates were analyzed for 3-nitrotyrosine immunoreactivity by Western blotting. ‘A’ is a representative blot of data obtained from 7 control and MCI samples, and ‘B’ is representative blot of data obtained from 5 control and AD samples, respectively.
(C) & (D) Graphical analysis of MCI and AD band intensities, respectively. The respective control values were set to 100%, to which experimental values were compared. Data are shown in arbitrary units on the ordinate axis as mean ±S.D. AD, #P<0.007.
