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. Author manuscript; available in PMC: 2008 Nov 25.
Published in final edited form as: Neuroscience. 2008 Mar 26;153(4):963–974. doi: 10.1016/j.neuroscience.2008.03.042

Fig. 2.

Fig. 2

ROS measurement by CM-H2DCFDA fluorescence in cortical neurons exposed to 300 µM KCN and various LED treatments for 24 h. A: Top panels: phase contrast; lower panels, the same fields as those in the top panels, but with CM-H2DCFDA fluorescence. CM-H2DCFDA expression was increased by KCN exposure, but the fluorescent signals were much lower in the two- and three- times-a-day LED-treated cells as compared to the others. Scale bar = 30 µm for all. B: Quantitative analyses of ROS expression. Two and three times of LED treatment exhibited significantly lower CM-H2DCFDA fluorescent values than those with other forms of LED treatment. LED once a day was not effective. All *P values were compared to controls: *, P < 0.05, ***, P < 0.001. +++, P < 0.001 compared to KCN alone.