Figure 2. LPS stimulation leads to CRH expression in the JEG3 trophoblast cells.
LPS, cHSP60 and IL1 stimulation of JEG3 cells induces CRH expression as assessed by RT-PCR (2A) and in JEG3 cells transiently transfected with a CRH-luciferase construct, stimulated with LPS for 24 hours, CRH promoter activation assessed by luciferase assay (2B). Test well luciferase levels were compared with those observed in the media treated control group. (*p< 0.05 as compared to media treated control).