Figure 3.

Inhibition of CaM kinase II α subunit expression results in epileptiform activity in hippocampal neurons in culture. (A) Representative intracellular recording from a missense-treated (control) neuron displaying background spontaneous synaptic activity (n = 10). An expanded record of spontaneous spike activity shows a single spike and no alteration of baseline polarity. (B) Representative intracellular recording from a neuron exposed to antisense oligonucleotides demonstrated epileptiform discharges and a high level of spontaneous synaptic activity (n = 10). An expanded record of three bursts shows numerous spikes superimposed on a large paroxysmal depolarization shift. [Standard bars: horizontal bar represents 2 min (slow sweep) and 2 s (fast sweep); and vertical bar represents 40 mV.]