. Author manuscript; available in PMC: 2009 Sep 1.

Published in final edited form as: Cell Signal. 2008 May 18;20(9):1616–1624. doi: 10.1016/j.cellsig.2008.05.004

Table 2. EC₅₀ of morphine and DAMGO for OPRM1 expressed in various MEF cells.

Wild type, βArr2^−/− and βArr1/2^−/− MEF cells were infected with adenovirus containing OPRM1. Morphine- and DAMGO-induced [Ca²⁺]_i release concentration-reponse curves were determined as described in Fig 1B. EC₅₀ values were calculated by log(agonist)vs. response formula using the data analysis program GraphPad Prism. Radioligand binding experments were performed as described in material and methods. Data were showed as the averages of n≥3 experiments.

MEF	Response		Receptor desensity, (pmol/mg)

	EC₅₀, Morphine, (nM)	EC₅₀ DAMGO , (nM)
WT	138 ± 48	30.5 ± 1.4	0.37 ± 0.08
βArr2−/−	194 ± 50	68.3 ± 2.2	0.34 ± 0.11
βArr1/2−/−	61 ± 5	25.3 ± 3.2	0.75 ± 0.23

means ± SEM (n ≥3)

Table 2. EC50 of morphine and DAMGO for OPRM1 expressed in various MEF cells.

Table 2. EC₅₀ of morphine and DAMGO for OPRM1 expressed in various MEF cells.