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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: J Cell Biochem. 2008 Nov 1;105(4):1008–1026. doi: 10.1002/jcb.21901

FIGURE 3. Activation of the human XOR promoter and upstream regulatory DNA in normal mouse and human MEC and in human BC cells.

FIGURE 3

A, B, ten individual clones of the XOR deletion set, spanning the upstream region from −1,900bp to −200bp, were transfected into mouse HC11 or human HB4a MEC respectively. Expression from each deletion was determined 48hrs after transfection. The parent expression vector, pGL3-Basic (pGL3-B) was transfected independently in each series. Note, the B10 designation, for example, corresponds to phXD-B10 and comprises the previously characterized XOR promoter, while B1 corresponds to phXD-B1 and comprises 1.9kbp of upstream regulatory DNA. C, the phXD-B1 clone containing 1.9kbp of XOR upstream regulatory DNA, including the proximal 200bp promoter, was transfected into the indicated cells at 1.0 to 5.0ug of DNA. Cells were co-transfected with 0.1ug of pcDNA3.1(+)HisMycLacZ and sufficient amounts of pGEM4 to bring the total mass of DNA to 5.1ug. Cells were harvested after 48hrs, and luciferase expression and lacZ expression were determined. Data were normalized to the amount of lacZ expression and are shown as normalized relative light units (R.L.U.).