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. Author manuscript; available in PMC: 2009 Nov 1.

Published in final edited form as: J Cell Biochem. 2008 Nov 1;105(4):1008–1026. doi: 10.1002/jcb.21901

A, pCMV-Myc-XOR or the pCMV-Myc empty vector were transfected into HC11-C24 in six well plates at the indicated DNA levels, cells were harvested after 24hrs, and oxypurinol inhibitable XOR activity was determined. B, pCMV-Myc-XOR or the pCMV-Myc empty vector were transfected into MDA-MB-231 in six well plates at the indicated DNA levels, cells were harvested after 24hrs, and oxypurinol inhibitable XOR activity was determined. Data show the mean and standard deviation of six independent determinations in both panels. C, western immunoblot of Myc-tagged XOR for the XOR activity data shown in panel B. D, Quantitation of migration in HC11-C24. pCMV-Myc-XOR or the pCMV-Myc empty vector were transfected into HC11-C24 in the presence or absence of the XOR inhibitor Y-700. The effect on migration was quantitated at 0, 19, and 31hrs later using open surface area determination. E, representative photomicrographs are shown for the migration assay in panel D. F, migration quantitation in MDA-MB-231. pCMV-Myc-XOR or the pCMV-Myc empty vector were transfected into MDA-MB-231 in the presence or absence of Y-700. The effect on migration was quantitated 0, 19, and 31hrs later by open surface area calculation. G, representative photomicrographs are shown for the wound assay in panel E.

A, pCMV-Myc-XOR or the pCMV-Myc empty vector were transfected into HC11-C24 in six well plates at the indicated DNA levels, cells were harvested after 24hrs, and oxypurinol inhibitable XOR activity was determined. B, pCMV-Myc-XOR or the pCMV-Myc empty vector were transfected into MDA-MB-231 in six well plates at the indicated DNA levels, cells were harvested after 24hrs, and oxypurinol inhibitable XOR activity was determined. Data show the mean and standard deviation of six independent determinations in both panels. C, western immunoblot of Myc-tagged XOR for the XOR activity data shown in panel B. D, Quantitation of migration in HC11-C24. pCMV-Myc-XOR or the pCMV-Myc empty vector were transfected into HC11-C24 in the presence or absence of the XOR inhibitor Y-700. The effect on migration was quantitated at 0, 19, and 31hrs later using open surface area determination. E, representative photomicrographs are shown for the migration assay in panel D. F, migration quantitation in MDA-MB-231. pCMV-Myc-XOR or the pCMV-Myc empty vector were transfected into MDA-MB-231 in the presence or absence of Y-700. The effect on migration was quantitated 0, 19, and 31hrs later by open surface area calculation. G, representative photomicrographs are shown for the wound assay in panel E.