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. Author manuscript; available in PMC: 2009 Nov 1.

Published in final edited form as: J Cell Biochem. 2008 Nov 1;105(4):1008–1026. doi: 10.1002/jcb.21901

A, HC11-C4, HC11-C24, and MDA-MB-231 cells were grown to confluency and treated with NECA at the indicated doses. XOR activity was determined 48hrs later. Data show the mean and standard deviation of four repetitions each. B, C, HC11-C4 MEC (B) or MDA-MB-231 (C) were grown to confluency and treated with either Y-700, N-acetylcysetine (NAC), or remained untreated. After one hour, cells were subjected to wounding, washed, and the medium refreshed in the presence of Y-700 or NAC, and NECA was added at the indicated doses. Migration was quantitated 19hrs (HC11-C4) or 48hrs (MDA-MB-231) after wounding using surface area calculation. Data show the mean and standard deviation of six independent assays. ***, p < 0.001 by Students T-test. D, E HC11-C4 (D) and MDA-MB-231 (E) cells were grown to confluency and treated with NAC at the indicated doses. After one hour, cells were subjected to wounding, washed, and the medium refreshed in the presence of NAC at the indicated doses. Migration was quantitated 19hrs (HC11-C4) or 48hrs (MDA-MB-231) after wounding using surface area calculation. Data show the mean and standard deviation of six independent assays. F, G HC11-C4 (F) and MDA-MB-231 (G) cells were grown to confluency and treated with urate at the indicated doses. After one hour, cells were subjected to wounding, washed, and the medium refreshed in the presence of urate at the indicated doses. Migration was quantitated 19hrs (HC11-C4) or 48hrs (MDA-MB-231) after wounding using surface area calculation. Data show the mean and standard deviation of six independent assays.

A, HC11-C4, HC11-C24, and MDA-MB-231 cells were grown to confluency and treated with NECA at the indicated doses. XOR activity was determined 48hrs later. Data show the mean and standard deviation of four repetitions each. B, C, HC11-C4 MEC (B) or MDA-MB-231 (C) were grown to confluency and treated with either Y-700, N-acetylcysetine (NAC), or remained untreated. After one hour, cells were subjected to wounding, washed, and the medium refreshed in the presence of Y-700 or NAC, and NECA was added at the indicated doses. Migration was quantitated 19hrs (HC11-C4) or 48hrs (MDA-MB-231) after wounding using surface area calculation. Data show the mean and standard deviation of six independent assays. ***, p < 0.001 by Students T-test. D, E HC11-C4 (D) and MDA-MB-231 (E) cells were grown to confluency and treated with NAC at the indicated doses. After one hour, cells were subjected to wounding, washed, and the medium refreshed in the presence of NAC at the indicated doses. Migration was quantitated 19hrs (HC11-C4) or 48hrs (MDA-MB-231) after wounding using surface area calculation. Data show the mean and standard deviation of six independent assays. F, G HC11-C4 (F) and MDA-MB-231 (G) cells were grown to confluency and treated with urate at the indicated doses. After one hour, cells were subjected to wounding, washed, and the medium refreshed in the presence of urate at the indicated doses. Migration was quantitated 19hrs (HC11-C4) or 48hrs (MDA-MB-231) after wounding using surface area calculation. Data show the mean and standard deviation of six independent assays.