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. 2008 Nov 14;105(47):18232–18237. doi: 10.1073/pnas.0809737105

Fig. 1.

Fig. 1.

Phenotypes and genotypes of 7 combinatorial transformants. (A) Endosperm colors of 7 different transgenic maize phenotypes. Ph-1 expressing Zmpsy1 only accumulates zeaxanthin and has a bright yellow color. Ph-2 expressing only PacrtI has a phenotype similar to WT M37W with a slight increase in total carotenoids. Ph-3 (Zmpsy1 and PacrtI) accumulates a significant amount of lycopene and has an orange–red color. Ph-4 expresses Gllycb in addition to Zmpsy1 and PacrtI and accumulates β-carotene, hence the orange color. Ph-5, Ph-6, and Ph-7 express Zmpsy1+PacrtI+Glbch, Zmpsy1+PacrtI+Gllycb, and Zmpsy1+PacrtI+Gllycb+Glbch, respectively, in addition to ParacrtW, thus showing a range of colors from orange to red depending on the accumulation of ketocarotenoids. (B) Northern blot analysis (30 μg of total RNA per lane) to monitor transgene expression in WT M37W and the 7 transgenic phenotypes (lanes 1–7). Staining of rRNA with ethidium bromide was used as a loading control.