Fig. 1.
ssDNA-dependent in vitro formation of the initiation complex TP-dAMP. The reaction mixtures contained, in addition to the indicated DNA polymerases (120 nM) and TPs (120 nM), 5.5 μM either ori(12)Nf or ori (12)φ oligonucleotides as templates. Reactions were started by adding 1 mM MnCl2 and, after incubation for 20 min at 30 °C, stopped, processed and analyzed by SDS-PAGE and autoradiography (see Materials and Methods for details). The various ssDNAs, DNA polymerases and TPs, and the mobility of the TP-dAMP complexes, are indicated. Only the area containing the relevant band is presented.