Table 2.
Real-time PCR confirmation about differentially expressed clones by >2-fold 2 h after 5-HT treatment in microarray
| EST name | BLASTX result* | GenBank accession number | Ratio of medians | Short description of real-time PCR results† |
|---|---|---|---|---|
| Up-regulated clones | ||||
| 5CAP092402_H04_760 | Matrilin (AAN61407, 1.00E-33) | EY418286 | 5.377 | Significant |
| 5CAP090501-pMES_D10_142 | Antistasin (P38977, 2.00E-25) | EY417467 | 4.830 | Significant |
| 5CAP031402_pMES_B12_1600 | Cathepsin L-like cysteine proteinase precursor (AAQ22984, 5.00E-05) | EY417083 | 4.419 | No change |
| 5CAP090504-pMES_E03_435 | alpha tubulin 2 (AAM09674, 6.00E-48) | EY417749 | 2.984 | No change |
| 5CAP092402_B10_694 | LOC443610 protein (AAI28922, 7.00E-55) | EY418226 | 2.838 | No change |
| ApC/EBP (positive control) | ApC/EBP (AAA18286, 0) | 2.594 | Significant | |
| 5HTCNS122105-T3-C10–628 | Eukaryotic translation initiation factor 3, subunit 6 (NP_001559, 5.00E-73) | EY416763 | 2.436 | Significant |
| 5CAP090501-pMES_F08_164 | Hypothetical protein (XP_001176996, 6.00E-33) | EY417489 | 2.230 | No change |
| Down-regulated clones | ||||
| 5CAP101001_F09_1029 | Kazal proteinase inhibitor (ABL74453, 2.00E-22) | EY418526 | 0.346 | No change |
| 5CAP120302-pMES_C06_1166 | unnamed protein product (CAG08644, 2.00E-14) | EY419713 | 0.356 | Reverse trend |
| 5CAP031405_pMES_G09_1891 | hCG1999844 (EAX04582, 0.013) | EY417337 | 0.460 | No change |
| 5CAP101001_H07_1051 | BAT1 homolog (AAQ13472, 2.00E-26) | EY418548 | 0.486 | Significant |
*BLASTX results are described as: gene description (accession number of BLASTX matched gene, E-value).
†Significant means P < 0.05 by student's t test; trend or reverse trend means 0.05 < P ≤ 0.10 by student's t test; no change means P > 0.10.