Table 3. Average NMJ areas and fibre diameters upon functional impairment of myosin Va.
FLAG | FLAG-MCLT | Scrambled silMyoVa | silMyoVa | |
NMJ area (µm2) | 706.8±22.7 | 475.7±18.6** | 753.4±28.2 | 426.5±23.3** |
Fibre diameter (µm) | 35.1±0.6 | 36.8±0.4 | 36.2±0.7 | 37.4±0.5 |
Number of fields (animals) | 8 (6) | 8 (4) | 7 (4) | 9 (4) |
TA muscles of adult wildtype mice were co-transfected with cytoplasmic YFP and FLAG, FLAG-MCLT, scrambled silMyoVa or silMyoVa, as indicated. Ten days later, transfected muscles were locally injected with BGT-AF647 and imaged with in vivo confocal microscopy. NMJ areas and diameters of corresponding fibres were determined. Data, mean±s.e.m.