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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Exp Gerontol. 2008 Aug 12;43(10):900–908. doi: 10.1016/j.exger.2008.08.005

Figure 4.

Figure 4

Ovariectomized (ovx) and sham females of the lubber grasshopper had converging levels of vitellogenin from 50 d to 200 d. Ovariectomized females had higher levels (LSmeans ± SE) of vitellogenin than did sham females at age 50 d, shortly after the median age of first oviposition in the sham females. Vitellogenin levels at age 100, 150, and 200 d were statistically similar in the two groups. In contrast, ovx and sham females tended to have divergent levels of hemolymph storage proteins from 50 d to 200 d. Storage protein levels in the two groups were similar at 50 d, while ovariectomized females had higher storage protein levels at 100 and 150 d, and marginally higher levels at 200 d (P = 0.054). Surgeries were performed during the first three days of adulthood. Vitellogenin levels were measured by ELISA (Borst et al. 2000).The combined levels of three hemolymph hexamerins (a conserved family of storage proteins) were estimated by measuring total hemolymph protein and subtracting vitellogenin (see Hatle et al. 2001).