Figure 3.

(A) Time-dependent changes in flash-induced changes in C_m at low time resolution. A flash application (arrow) elicited a transient in [Ca²⁺]_i (Bottom). Dashed line represents resting C_m of 4.2 pF [resting C_m was 5.3 ± 0.2 pF (mean ± SEM, n = 50)]. The maximum amplitude of the Ca²⁺-induced rise in C_m (slow exocytosis) is indicated by A. G shows conductance, which reflects changes in access conductance, membrane conductance, and C_m. Access conductance (140 nS, dashed line) was determined with controls on the SWAM amplifier. (B) Expanded high time resolution of C_m trace from A, labeled by *. Changes in C_m after a flash (arrow)-induced increase in [Ca²⁺]_i (Lower) and C_m (Upper). B indicates the amplitude of the early rapid C_m response.