Figure 1. MyD88^−/− mice are highly susceptible to rMA15 infection and experience higher levels of virus replication within the lung as compared to WT mice.

10 week-old C57BL/6 WT (n = 14), RAG-1^−/− (n = 21), or MyD88^−/− (n = 16) mice were infected intranasally with 10⁵ pfu rMA15. (A) Mice were monitored for changes in body weight at 24 h intervals. Each data point represents the mean ± the standard deviation. Asterisks indicate statistical significance by the non-parametric Mann-Whitney test with a P<0.05. (B) Mice were monitored for mortality. Data is expressed as a percent survival value. (C) WT, MyD88^−/− or RAG-1^−/− mice were infected intranasally with 10⁵ pfu rMA15. Lung tissues from 3–6 mice per strain were harvested on 1, 3, 4, 6, and 9 dpi. Lung tissues were homogenized in DPBS and virus titers within clarified supernatants were assessed by plaque assay. Mean titers are displayed and error bars represent 1 standard deviation. *, P<0.05 as determined by the Mann-Whitney test.