Figure 4.

RNase H cleavage of a DOR-2 RNA target sequence with DNA and DNA/LNA copolymers. (Upper) RNase H cleavage of a 15-mer synthetic RNA (5′UGCCUUCUGCCCGUG) lowercase letters corresponding to the DOR-2 target. (Right) Control incubations lacking added antisense DNA or RNase H, thus demonstrating that the degradation visible (Upper) is mediated by added RNase H and antisense DNA. LNA monomers are represented by uppercase letters, DNA monomers by lowercase letter, and phosphorothioate (PS) monomers by italic lowercase letters. The location of the full-length RNA is indicated (arrow), and the relative volume densities for single measurements are shown as line graphs.