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. 2008 Dec;19(12):2321–2330. doi: 10.1681/ASN.2007111200

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Copyright © 2008 by the American Society of Nephrology

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Figure 3. — Exocytosis of WPB induced by UA. (A and B) Treatment of HUVEC with MSU (100 μg/ml) resulted in a rapid exocytosis of WPB and the loss of cellular vWF and angiopoietin 2. Images were obtained and processed as detailed in the Concise Methods section. Quantitative analysis of fluorescence intensity for A. Approximately 100 individual cells were analyzed in each of three independent experiments. (C and D) Dosage dependence (10, 50, and 100 μg/ml) of MSU effect on the expression of angiopoietin 2 and vWF. Quantitative analysis of fluorescence intensity. *Significant decrease in the expression of vWF and angiopoietin 2.