Figure 3.

CNI downregulate the expression of CXCR3-B in human renal cancer cells. (A through C) REC, 786-0, and Caki-1 cells were treated with CsA (0.1 and 1.0 μg/ml), FK506 (0.01 and 0.10 μg/ml), or vehicle alone and incubated for 18 h. Total RNA was isolated from these cells and reverse-transcribed. Fold changes in mRNA expression of CXCR3-B in response to CsA (A) or FK506 (B) was measured by real-time PCR. Fold changes in mRNA expression of CXCR3-A in response to CsA was also measured by real-time PCR (C). Data reflect three independent experiments. Columns are average of triplicate readings of two different samples; bars are ±SD. In A and B, *P < 0.05 versus vehicle-treated cells.