Figure 6.

CNI-mediated proliferation of renal cancer cells involves G_i proteins. (A) Caki-1 cells were transfected with either the CXCR3-B or control siRNA (50 nM) for 48 h, incubated with PTX (1.0 μg/ml)/vehicle for 16 h, and then either untreated or treated with CXCL10 (20 ng/ml) for 24 h. (B) 786-0 cells were incubated with PTX (1.0 μg/ml)/vehicle for 16 h and then treated with either CsA (1.0 μg/ml) or vehicle alone for 72 h. All of the cells in A and B were subjected to cell proliferation assay by measuring [³H]thymidine incorporation within the cells as described in the Concise Methods section. Data reflect three independent experiments. Columns are average of triplicate readings (cpm) of the samples; bars are ±SD. In A and B, *P < 0.05 versus either control siRNA-transfected or vehicle-treated cells; in A, ⁺P < 0.05 versus CXCR3-B siRNA-transfected but PTX-untreated cells and ^#P < 0.05 versus control siRNA-transfected untreated cells; in B, ⁺P < 0.05 versus CsA-treated but PTX-untreated cells.