Figure 5.

Mean (+SEM) CRH mRNA (Upper) and AVP mRNA (Lower) in the PVN of adult SALINE, ENDO, and HANDLED rats 14 days after AA or vehicle injection. mRNA was measured as optical density arbitrary units relative to standard values from autoradiograph images. In situ hybridizations were performed with oligonucleotide probes corresponding to rat CRH or AVP mRNA coding sequences. CRH mRNA was reduced in all neonatal treatment conditions in animals injected with adjuvant; *, P < 0.05. Control levels of CRH mRNA were greater in the ENDO animals relative to HANDLED animals; **, P < 0.05. An increase in AVP mRNA expression was observed in all AA experimental groups by day 14 after injection compared with Vehicle controls; *, P < 0.05. Overall, AVP mRNA levels were greater in ENDO animals compared with the other neonatal treatment conditions; **, P < 0.05.