Figure 2.
The Gi/oα subunits have similar affinities in mediating signaling between A1 receptors and Kir3.1+3.2A. (A) Superimposed dose-response curves for NECA-induced activation of Kir3.1+3.2A channels in control, non-PTx treated cells (solid line) and in PTx-treated cells in which the mutant Gi/oα subunits (dashed lines), Giα1C351G, Giα2C352G, Giα3C351G, and GoαAC351G, have been co-expressed. (B) Bar chart summarizing the data obtained with the HKIR3.1/3.2/A1 cell line and expression of each of the Gi/o variants. Open bars represent basal currents, and solid bars represent current in response to receptor stimulation. Numbers in parentheses refer to the number of cells recorded from for each experiment. Current density was measured at −100 mV.