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Western blot demonstrating VEGF protein expression in the ipsilateral temporal muscle (A) and in the brain (B) after the encephalomyosynangiosis operation. A single band around 45 Kd is detected with rabbit polyclonal antibody against VEGF in the brain, and a double band is detected around 40 Kd in muscle. Both muscles and brain tissue show no definite difference in levels of expression. VEGF : vascular endothelial growth factor.
