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. Author manuscript; available in PMC: 2008 Nov 26.
Published in final edited form as: J Biol Chem. 2007 Jul 23;282(38):27562–27571. doi: 10.1074/jbc.M700340200

Figure 5. Over-expression of GBS NeuA decreases Sia-O-acetylation on GBS strains of multiple serotypes.

Figure 5

WT GBS strains were transformed with an expression construct encoding the full-length NeuA enzyme “+A” and O-acetylation was compared to the parent strain. Total cellular Sias were isolated by mild acid hydrolysis and fluorescently derivatized with DMB as described in Experimental Procedures. Derivatized Sias were resolved by reverse phase HPLC and percent O-acetylation determined by software-assisted integration of HPLC peaks corresponding to O-acetylated and non-O-acetylated Neu5Ac peaks. HPLC profiles of the COH1 strain and corresponding NeuA-overexpressing strain were published previously (17).