Table 1.
FACS analysis of lymphocyte subpopulations in conventional and germ-free Balb/c mice fed either a low LPS diet (AIN-93G) or a LPS-rich diet (ST1)
| Number of positive lymphocytes (× 106) | ||||
| Lymphoid organ/tissue | Subpopulation | CV ST1 | GF ST1 | GF AIN |
| Peyer's patches | Total | 4.69 ± 0.16 | 1.57 ± 0.19 | 0.67 ± 0.09 |
| CD19+ | 2.93 ± 0.10 | 1.00 ± 0.12 | 0.40 ± 0.05 | |
| CD3+ | 1.38 ± 0.05 | 0.44 ± 0.05 | 0.21 ± 0.03 | |
| CD4+ | 0.95 ± 0.03 | 0.33 ± 0.04 | 0.16 ± 0.02 | |
| CD8+ | 0.27 ± 0.01 | 0.08 ± 0.01 | 0.04 ± 0.01 | |
| CD4+Foxp3+ | 0.15 ± 0.01 | 0.04 ± 0.01 | 0.02 ± 0.00 | |
| CD8+Foxp3+ | 0.02 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | |
| Mesenteric lymph nodes | Total | 17.81 ± 0.69 | 7.82 ± 0.57 | 4.96 ± 0.96 |
| CD19+ | 4.39 ± 0.17 | 2.96 ± 0.21 | 2.48 ± 0.48 | |
| CD3+ | 12.10 ± 0.47 | 4.70 ± 0.34 | 2.36 ± 0.46 | |
| CD4+ | 8.70 ± 0.34 | 3.40 ± 0.25 | 1.53 ± 0.30 | |
| CD8+ | 2.41 ± 0.09 | 1.03 ± 0.07 | 0.64 ± 0.12 | |
| CD4+Foxp3+ | 1.42 ± 0.05 | 0.48 ± 0.04 | 0.22 ± 0.04 | |
| CD8+Foxp3+ | 0.06 ± 0.00 | 0.02 ± 0.00 | 0.01 ± 0.00 | |
| Spleen | Total | 83.14 ± 5.28 | 62.48 ± 3.46 | 66.21 ± 4.12 |
| CD19+ | 46.14 ± 2.93 | 35.59 ± 1.97 | 36.44 ± 2.27 | |
| CD3+ | 24.74 ± 1.57 | 20.54 ± 1.14 | 16.93 ± 1.05 | |
| CD4+ | 16.32 ± 1.04 | 12.35 ± 0.68 | 11.18 ± 0.70 | |
| CD8+ | 7.00 ± 0.44 | 5.36 ± 0.30 | 5.31 ± 0.33 | |
| CD4+Foxp3+ | 3.03 ± 0.19 | 2.16 ± 0.12 | 2.27 ± 0.14 | |
| CD8+Foxp3+ | 0.12 ± 0.01 | 0.12 ± 0.01 | 0.11 ± 0.01 | |
| Peritoneal cells | Total | 2.55 ± 0.41 | 2.65 ± 0.06 | 2.50 ± 0.35 |
| CD19+ | 1.80 ± 0.29 | 2.22 ± 0.05 | 2.19 ± 0.30 | |
| CD3+ | 0.11 ± 0.02 | 0.08 ± 0.00 | 0.07 ± 0.01 | |
| CD4+ | 0.22 ± 0.04 | 0.18 ± 0.00 | 0.16 ± 0.02 | |
| CD8+ | 0.06 ± 0.01 | 0.06 ± 0.00 | 0.05 ± 0.01 | |
| CD4+Foxp3+ | 0.03 ± 0.01 | 0.03 ± 0.00 | 0.03 ± 0.00 | |
| CD8+Foxp3+ | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | |
| Thymus | Total | 207.03 ± 9.23 | 205.76 ± 8.46 | 205.76 ± 7.98 |
| CD4-CD8- | 8.70 ± 0.30 | 10.72 ± 0.50 | 11.87 ± 0.20 | |
| CD4+CD8+ | 155.27 ± 8.13 | 151.59 ± 7.26 | 146.52 ± 7.18 | |
| CD4+CD8- | 30.23 ± 1.82 | 28.81 ± 1.72 | 31.39 ± 2.39 | |
| CD8+CD4- | 13.04 ± 0.67 | 11.93 ± 0.57 | 9.88 ± 0.57 | |
| CD4+Foxp3+ | 1.35 ± 0.05 | 1.25 ± 0.03 | 1.15 ± 0.02 | |
| CD8+Foxp3+ | 0.66 ± 0.02 | 0.66 ± 0.02 | 0.65 ± 0.01 | |
The single cell suspensions were stained with the monoclonal antibodies as described under "Methods" and analyzed by FACS. PP and MLN cell numbers represent the overall cell pool isolated from one mouse. Data is a summary of at least four independent experiments (at least 10 mice/group) and values represent the mean ± SE.