Table 1.

Genes selected for expression analysis

Gene Name	Gene Symbol	Cellular Function	Primer Sequence (5'-3')	Reference	Accession #
bactin1	bactin1	Cytoskeleton	F) CGAGCAGGAGATGGGAACC	14	AF057040
			R) CAACGGAAACGCTCATTGC
tubulin, alpha 1	tuba1	Cytoskeleton	F) CCTGCTGGGAACTGTATTGT	*	AF029250
			R) TCAATGAGTTCCTTGCCAAT
glyceraldehyde-3-phosphate dehydrogenase	gapdh	Glycolysis enzyme	F) GTGGAGTCTACTGGTGTCTTC	15	BC083506
			R) GTGCAGGAGGCATTGCTTACA
glucose-6-phosphate dehydrogenase	g6pd	Glycolysis enzyme	F) GTCCCGAAAGGCTCCACTC	9	BM182602
			R) CCTCCGCTTTCCTCTC
TATA-box-binding protein	tbp	Transcription	F) CGGTGGATCCTGCGAATTA	*	NM_200096
			R) TGACAGGTTATGAAGCAAAACAACA
beta-2-microglobulin	b2m	Major histocompatibility complex	F) GCCTTCACCCCAGAGAAAGG	*	BC062841
			R) GCGGTTGGGATTTACATGTTG
elongation factor 1-alpha	elfa	Translation	F) CTTCTCAGGCTGACTGTGC	16	AY422992
			R) CCGCTAGCATTACCCTCC
18s ribosomal RNA	18s	Ribosome subunit	F) TCGCTAGTTGGCATCGTTTATG	17	BX296557
			R) CGGAGGTTCGAAGACGATCA
cytochrome P450, family 19, subfamily A, polypeptide 1b	cyp19a1b	Steroid biosynthesis	F) AAAGAGTTACTAATAAAGATCCACCGGTAT	13	AF226619
			R) TCCACAAGCTTTCCCATTTCA
cytochrome P450, family 1, subfamily A	cyp1a	Xenobiotic metabolism	F) GCATTACGATACGTTCGATAAGGAC	18	NM_131879
			R) GCTCCGAATAGGTCATTGACGAT

The first 8 were candidate housekeeping genes. cyp19a1b and cyp1a, targets of ER and AhR mediated signal transduction, respectively, were used to tests effects of normalization on apparent gene expression. Asterisks (*) indicate primers designed in this study.