Table 2.

Housekeeping gene expression in different tissues of adult male (M) and female (F) zebrafish, as measured by QPCR

Ct Values +/- S.E.M.
	EYE		BRAIN		HEART		LIVER		MUSCLE		GONAD
GENE	M	F	M	F	M	F	M	F	M	F	M	F
bactin1	16.2 ± 0.45	16.4 ± 0.26	16.4 ± 0.33	16.4 ± 0.46	17.5 ± 0.33	16.7 ± 0.57	18.2 ± 0.42	17.7 ± 0.30	17.7 ± 0.58	16.8 ± 0.51	16.2 ± 0.29	14.2 ± 0.24
tuba1	23.2 ± 0.19	23.1 ± 0.17	22.2 ± 0.23	22.3 ± 0.18	22.3 ± 0.29	23.5 ± 0.43	27.8 ± 0.28	29.2 ± 0.27	27.0 ± 0.40	25.4 ± 0.18	21.2 ± 0.30	21.4 ± 0.85
gapdh	18.4 ± 0.33	17.8 ± 0.58	22.6 ± 0.98	23.6 ± 0.84	15.0 ± 0.35	15.2 ± 0.53	15.8 ± 0.62	16.8 ± 0.62	15.4 ± 0.07	14.8 ± 0.34	20.1 ± 0.78	15.0 ± 0.45
g6pd	21.2 ± 0.08	21.6 ± 0.17	21.8 ± 0.11	22.0 ± 0.38	22.2 ± 0.07	21.4 ± 0.19	20.3 ± 0.20	21.0 ± 0.12	25.1 ± 0.37	23.3 ± 0.11	20.4 ± 0.10	19.1 ± 0.16
tbp	22.1 ± 0.24	22.6 ± 0.25	21.9 ± 0.19	21.7 ± 0.17	24.1 ± 0.26	22.2 ± 0.29	24.0 ± 0.16	23.7 ± 0.08	24.6 ± 0.19	20.6 ± 0.16	20.2 ± 0.03	16.5 ± 0.15
b2m	17.6 ± 0.05	16.9 ± 0.03	18.2 ± 0.16	17.1 ± 0.01	15.9 ± 0.14	15.8 ± 0.04	16.1 ± 0.01	16.7 ± 0.01	17.4 ± 0.07	16.7 ± 0.29	16.0 ± 0.06	18.4 ± 0.22
elfa	15.2 ± 0.08	15.3 ± 0.02	15.7 ± 0.02	15.7 ± 0.27	14.8 ± 0.05	14.9 ± 0.18	14.3 ± 0.08	14.2 ± 0.04	16.7 ± 0.11	16.0 ± 0.04	14.6 ± 0.09	14.1 ± 0.03
18s	16.6± 0.07	16.6 ± 0.08	16.7 ± 0.05	16.9 ± 0.05	17.6 ± 0.13	17.0 ± 0.06	16.5 ± 0.08	16.4 ± 0.11	17.3 ± 0.10	16.8 ± 0.08	16.6 ± 0.07	17.0 ± 0.34

Values represent Ct values (mean +/- SEM) of tissues of each type pooled by sex (3 independent pools per tissue type/sex/5 fish per pool). For details, see Methods and Results. Boldface indicates significant difference between male and female of the same tissue by t-test p < .05. All genes showed significant differences across tissue types by one-way ANOVA p < .05.