Table 1.
a) Amplification success1 | b) Polymorphism2 | ||||||||||
Error | Residual | B | t | P | Error | Residual | B | t | P | ||
df * | deviance* | df * | deviance* | ||||||||
Maximal model | 39 | 57.29 | Maximal model | 12 | 24.97 | ||||||
- Interactions | 49 | 62.32 | - Microsatellite retained | 13 | 24.97 | ||||||
- Repeat length | 50 | 62.32 | - Primer type5 | 14 | 25.46 | ||||||
- Unique/multiple hits | 51 | 62.70 | - Ho in source species6 | 17 | 26.91 | ||||||
- Microsatellite motif3 | 52 | 63.55 | - No. of mismatches7 | 18 | 27.60 | ||||||
- No of mismatches4 | 53 | 67.65 | - E-value | 19 | 30.15 | ||||||
Minimal adequate model | Minimal adequate model | ||||||||||
E-value | 53 | 67.65 | -0.02 | -2.58 | 0.013 | Repeat length | 19 | 30.15 | 0.02 | 2.22 | 0.039 |
Microsatellite motif3 | 19 | 30.15 | -1.00 | -2.15 | 0.045 | ||||||
Microsatellite interrupted8 | 19 | 30.15 | -1.02 | -2.09 | 0.051 |
*model descriptives are given for models after removal of explanatory variables
1based on tests in three charadriiform species: Kentish plover Charadrius alexandrinus, whiskered auklet Aethia pygmaea and ruff Philomachus pugnax
2based on proportion of species in which a microsatellite locus was found to be polymorphic when tested in four individuals
3dinucleotide or tetranucleotide repeat motif of microsatellite
4total number of mismatches between chicken and charadriiform sequence for both primer sequences combined
5Gga-consensus primer or standard primer design
6observed heterozygosity for source species given in original publication of the microsatellite sequence
7number of mismatches in primer sequence between charadriiform sequence and chicken homologue
8microsatellite sequence interrupted by non-motif base insertions (imperfect) or not (perfect)