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. 2008 Oct 24;9:502. doi: 10.1186/1471-2164-9-502

Table 1.

Generalised linear models for a) amplification success and b) polymorphism of conserved microsatellite loci

a) Amplification success1 b) Polymorphism2
Error Residual B t P Error Residual B t P
df * deviance* df * deviance*
Maximal model 39 57.29 Maximal model 12 24.97
- Interactions 49 62.32 - Microsatellite retained 13 24.97
- Repeat length 50 62.32 - Primer type5 14 25.46
- Unique/multiple hits 51 62.70 - Ho in source species6 17 26.91
- Microsatellite motif3 52 63.55 - No. of mismatches7 18 27.60
- No of mismatches4 53 67.65 - E-value 19 30.15
Minimal adequate model Minimal adequate model
E-value 53 67.65 -0.02 -2.58 0.013 Repeat length 19 30.15 0.02 2.22 0.039
Microsatellite motif3 19 30.15 -1.00 -2.15 0.045
Microsatellite interrupted8 19 30.15 -1.02 -2.09 0.051

*model descriptives are given for models after removal of explanatory variables

1based on tests in three charadriiform species: Kentish plover Charadrius alexandrinus, whiskered auklet Aethia pygmaea and ruff Philomachus pugnax

2based on proportion of species in which a microsatellite locus was found to be polymorphic when tested in four individuals

3dinucleotide or tetranucleotide repeat motif of microsatellite

4total number of mismatches between chicken and charadriiform sequence for both primer sequences combined

5Gga-consensus primer or standard primer design

6observed heterozygosity for source species given in original publication of the microsatellite sequence

7number of mismatches in primer sequence between charadriiform sequence and chicken homologue

8microsatellite sequence interrupted by non-motif base insertions (imperfect) or not (perfect)