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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Mol Cancer Ther. 2008 Aug;7(8):2445–2454. doi: 10.1158/1535-7163.MCT-08-0063

Figure 1. HLI373, a highly soluble derivative of HLI98, is more potent than HLI98s.

Figure 1

Figure 1

A, Structure of HLI98 family and HLI373. HLI98C: R2 = Cl, HLI98D: R1 = Cl, HLI98E: R1 = CH3. B, RPE cells were treated with 1 μg/ml Adriamycin (Adr), 50 μM MG132 (MG), or HLI373 dissolved in PBS or DMSO and added to cells at the indicated final concentrations for 8 h. Levels of p53, Hdm2 and β-actin, used as a loading control, were assessed by immunoblotting with antibodies directed against the indicated proteins. C, RPE cells were incubated with 1 μg/ml Adriamycin, 50 μM ALLN, or the indicated concentrations of HLI373 for 8 h followed by assessment as in B. D, Immobilized bacterially-expressed GST-Hdm2 was treated as indicated prior to carrying out an in vitro auto-ubiquitylation reaction using 32P-labeled ubiquitin. Lack of E1 in the reaction mixture served as a negative control.