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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: Alcohol Clin Exp Res. 2008 Aug 20;32(11):1884–1892. doi: 10.1111/j.1530-0277.2008.00769.x

Fig 2. PARP-1 expression is enhanced with ethanol treatment.

Fig 2

Neurons grown on cover slips were treated with 4mg/ml ethanol for 24 hours. Immunofluorescent staining was done using anti-PARP-1 antibody followed by Cy-3 conjugated rabbit secondary antibody. There is a robust increase in PARP-1 expression in ethanol exposed samples, primarily in the nuclei (Fig. 2A). Fig. 2B is a composite of five random fields. The intensity increase is approximately 10 fold (n=5, *= p<0.0002).