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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: Alcohol Clin Exp Res. 2008 Aug 20;32(11):1884–1892. doi: 10.1111/j.1530-0277.2008.00769.x

Fig 4. PARP-1 is inactivated by caspase-3.

Fig 4

PARP-1 is cleaved from a full length 113kDa during apoptosis to generate an 89- and a 24kDa fragment. To detect the cleavage of PARP-1 an antibody was used that recognizes the N-terminal 24kDa fragment. With increased duration of ethanol exposure (4, 12 and 24 hours), there are increases in PARP-1 which reach statistical significance (p<0.05) by 24 hours of exposure. Caspase inhibition with zVAD during the 24 hour ethanol (4mg/ml) treatment reduced expression of the 24kDA band. Data represents n=3 experiments. (* = p< 0.05)