Fig 5. Ethanol increases translocation of AIF from mitochondria to the nuclear compartment.
Neurons were treated with E (4 mg/ml) for 2, 6 and 24 hours. The expressions of AIF were detected by western blotting using anti-AIF primary antibodies. The primary antibodies were detected using HRP-conjugated secondary antibodies followed by use of a chemiluminescence reagent kit (ECL). Data represents n=3 experiments