Table 1.
LeETR expression and ethylene synthesis in leaves of NR and LeETR4 antisense lines
| Line | LeETR1 mRNA, % | LeETR2 mRNA, % | LeETR5 mRNA, % | Ethylene, nl/gfw/hr |
|---|---|---|---|---|
| Wild type | 1.3 ± 0.1 × 10−3 | 3.2 ± 1.8 × 10−4 | 4.1 ± 0.1 × 10−4 | 3.3 ± 0.3 |
| NRAS-1 | 1.5 ± 0.2 × 10−3 | 5.3 ± 1.5 × 10−4 | 6.9 ± 0.2 × 10−4** | 3.1 ± 0.4 |
| NRAS-2 | 1.4 ± 0.4 × 10−3 | 5.0 ± 1.2 × 10−4 | 6.8 ± 1.8 × 10−4 | 2.9 ± 0.5 |
| NRAS-3 | 1.2 ± 0.1 × 10−3 | 4.5 ± 1.7 × 10−4 | 5.2 ± 1.7 × 10−4 | 3.1 ± 0.5 |
| Wild type | 7.5 ± 2.9 × 10−4 | 3.9 ± 0.3 × 10−4 | 1.6 ± 0.4 × 10−3 | 4.6 ± 0.7 |
| LeETR4AS-1 | 7.3 ± 3.2 × 10−4 | 4.0 ± 1.4 × 10−4 | 1.9 ± 0.6 × 10−3 | 4.8 ± 0.7 |
| LeETR4AS-2 | 9.0 ± 2.1 × 10−4 | 4.5 ± 0.6 × 10−4 | 1.2 ± 0.3 × 10−3 | 5.4 ± 0.6 |
| LeETR4AS-3 | 5.5 ± 1.0 × 10−4 | 2.6 ± 0.3 × 10−4* | 1.3 ± 0.4 × 10−3 | 6.0 ± 0.7 |
LeETR mRNA levels expressed as % of total mRNA were determined as described in Methods. Numbers represent means ± SE.
*Significantly different from wild type (P ≤ 0.05).
**Significantly different from wild type (P ≤ 0.01).